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The Detection of 5-Hydroxymethylcytosine in Neural Stem Cells and Brains of Mice
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神经科学
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JoVE 杂志 神经科学
The Detection of 5-Hydroxymethylcytosine in Neural Stem Cells and Brains of Mice

The Detection of 5-Hydroxymethylcytosine in Neural Stem Cells and Brains of Mice

DOI:
10.3791/59950-v

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08:03 min

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September 19, 2019

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, , , ,
1The Children’s Hospital, School of Medicine,Zhejiang University, 2The Institute of Translational Medicine, School of Medicine,Zhejiang University

Chapters

  • 00:04Title
  • 01:00Transcardial Perfusion of the Mouse
  • 02:03Brain Sectioning and Immunofluorescence Staining
  • 04:02Genomic DNA Isolation
  • 05:43DNA Dot Blot
  • 07:03Results: Immunofluorescence Staining and Dot Blot Detection
  • 07:49Conclusion

Summary

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Here, we present a protocol to detect 5-hydroxymethylcytosine in cells and brain tissues, utilizing immunofluorescence staining and DNA dot-blot methods.

Tags

Keywords: 5-hydroxymethylcytosine5hmCImmunofluorescenceDNA Dot-blotNeural Stem CellsMouse BrainEpigenetic ModificationGene ExpressionNeurological DisordersPerfusionFixationCryosectioningImmunostaining

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