Journal
/
Chemistry
/
NMR-Based Activity Assays for Determining Compound Inhibition, IC50 Values, Artifactual Activity, and Whole-Cell Activity of Nucleoside Ribohydrolases
JoVE Journal
Chemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Chemistry
NMR-Based Activity Assays for Determining Compound Inhibition, IC50 Values, Artifactual Activity, and Whole-Cell Activity of Nucleoside Ribohydrolases

NMR-Based Activity Assays for Determining Compound Inhibition, IC50 Values, Artifactual Activity, and Whole-Cell Activity of Nucleoside Ribohydrolases

English

Automatically Generated

9,778 Views

•

10:24 min

•

June 30, 2019

DOI:

10.3791/59928-v

DOI:
10.3791/59928-v

•

10:24 min
June 30, 2019

•

5 Views
, , , , , , , , , , ,
1Department of Chemistry,Adelphi University, 2Department of Chemistry,Washington University in St. Louis, 3Department of Chemistry,Boston University

Explore More Videos

NMR-based Activity AssaysEnzyme InhibitionIC50 ValuesArtifact DetectionWhole-cell ActivityNucleoside RibohydrolasesFragment ScreeningTrichomonas VaginalisAnti-trichomonal DrugsReagent VolumesReaction ComponentsSubstrate PreparationTest Compound PreparationReaction Stock SolutionReaction InitiationReaction Quenching

Read Article

Cite this Article

Stockman, B. J., Kaur, A., Persaud, J. K., Mahmood, M., Thuilot, S. F., Emilcar, M. B., Canestrari, M., Gonzalez, J. A., Auletta, S., Sapojnikov, V., Caravan, W., Muellers, S. N. NMR-Based Activity Assays for Determining Compound Inhibition, IC50 Values, Artifactual Activity, and Whole-Cell Activity of Nucleoside Ribohydrolases. J. Vis. Exp. (148), e59928, doi:10.3791/59928 (2019).

Download .ris file

Copy

Share Video

.

Copy