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Beoordeling van oxidatieve schade in de primaire muis oculaire oppervlaktecellen / stamcellen in reactie op Ultraviolet-C (UV-C) Schade
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JoVE Journal Developmental Biology
Assessment of Oxidative Damage in the Primary Mouse Ocular Surface Cells/Stem Cells in Response to Ultraviolet-C (UV-C) Damage
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Beoordeling van oxidatieve schade in de primaire muis oculaire oppervlaktecellen / stamcellen in reactie op Ultraviolet-C (UV-C) Schade

Nederlands

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5,882 Views

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12:59 min

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February 15, 2020

DOI:

10.3791/59924-v

DOI:
10.3791/59924-v

•

12:59 min
February 15, 2020

•

4 Views
, , , , ,
1Stem Cells and Regenerative Medicine Centre,Yenepoya Research Centre, Yenepoya (Deemed to be University), Mangaluru-575018, Karnataka, India

Summary

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Dit protocol toont de gelijktijdige detectie van reactieve zuurstofsoorten (ROS), levende cellen en dode cellen in levende primaire culturen van muisoculaire oppervlaktecellen. 2',7'-Dichloorfluoresceindiacetaat, propidiumjodide en Hoechst-vlekken worden gebruikt om respectievelijk de ROS-, dode cellen en levende cellen te beoordelen, gevolgd door beeldvorming en analyse.

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Oxidative DamagePrimary Mouse Ocular Surface CellsStem CellsUltraviolet-C (UV-C) DamageReactive Oxygen Species (ROS)DCFDAPropidium IodideHoechstLive Cell PermeantDead Cell PermeantUV CrosslinkerDose-response

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Cite this Article

Bose, B., Kapoor, S., Sen, U., Nihad AS, M., Chaudhury, D., Shenoy P, S. Assessment of Oxidative Damage in the Primary Mouse Ocular Surface Cells/Stem Cells in Response to Ultraviolet-C (UV-C) Damage. J. Vis. Exp. (156), e59924, doi:10.3791/59924 (2020).

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