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Gene direto knock-out de células-tronco neurais da medula espinhal Axolotl via eletroporação de complexos de proteína-gRNA CAS9
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Genetics
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JoVE Journal Genetics
Direct Gene Knock-out of Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes

Gene direto knock-out de células-tronco neurais da medula espinhal Axolotl via eletroporação de complexos de proteína-gRNA CAS9

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7,821 Views

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09:10 min

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July 09, 2019

DOI:

10.3791/59850-v

DOI:
10.3791/59850-v

•

09:10 min
July 09, 2019

•

3 Views
, , , ,
1School of Life Sciences,South China Normal University, 2The Research Institute of Molecular Pathology (IMP),Vienna Biocenter (VBC), 3Institute for Brain Research and Rehabilitation (IBRR),South China Normal University

Summary

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É apresentado aqui um protocolo para executar a batida-para fora do gene tempo-e espaço-restrito em cordas espinais do Axolotl injetando o complexo de CAS9-gRNA no canal central da medula espinal seguido pelo Electroporation.

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Cite this Article

Lou, W. P., Wang, L., Long, C., Liu, L., Fei, J. Direct Gene Knock-out of Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes. J. Vis. Exp. (149), e59850, doi:10.3791/59850 (2019).

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