Direct Gene Knock-out of Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes

Wilson Pak-Kin Lou; Liqun Wang; Cheng Long; Lei Liu; Ji-Feng Fei

doi:10.3791/59850

Journal

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Genetics

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Direct Gene Knock-out of Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes

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Genetics

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JoVE Journal Genetics

Direct Gene Knock-out of Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes

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Direct Gene Knock-out of Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes

7,821 Views

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09:10 min

•

July 09, 2019

DOI:

10.3791/59850-v

DOI:

10.3791/59850-v

•

09:10 min

July 09, 2019

•

3 Views

Wilson Pak-Kin Lou*^1,2, Liqun Wang*³, Cheng Long¹, Lei Liu³, Ji-Feng Fei³

¹School of Life Sciences,South China Normal University, ²The Research Institute of Molecular Pathology (IMP),Vienna Biocenter (VBC), ³Institute for Brain Research and Rehabilitation (IBRR),South China Normal University

Summary

Présenté ici est un protocole pour effectuer le temps et l'espace-limité gène knock-out dans les moelles épinières axolotl en injectant CAS9-gRNA complexe dans le canal central de la moelle épinière suivie d'électroporation.

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Direct Gene Knock-out of Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes

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Summary

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