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Aislamiento de células mononucleares lamina Propria de Colon Murino usando colagenasa E
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1Department of Pediatrics, Division of Hematology / Oncology and Bone Marrow Transplantation,University of Miami Miller School of Medicine, 2Batchelor Children’s Research Institute,University of Miami Miller School of Medicine, 3Department of Microbiology & Immunology,University of Miami Miller School of Medicine, 4Sylvester Comprehensive Cancer Center,University of Miami Miller School of Medicine, 5Holtz Children’s Hospital,University of Miami Miller School of Medicine
Chapters
- 00:04Title
- 01:00Harvesting the Colon
- 01:32Cleaning the Colon
- 03:10Collagenase Digestion 1
- 03:34Silica-Based Density Separation Media Gradient Separation
- 05:44Collect Mononuclear Cells from the Gradient Interface
- 06:39Results: Isolation of Lamina Propria Mononuclear Cells
- 08:40Conclusion
El objetivo de este protocolo es aislar las células mononucleares que residen en la lámina propria del colon mediante la digestión enzimática del tejido utilizando la colagenasa. Este protocolo permite el aislamiento eficiente de las células mononucleares, lo que resulta en una suspensión de una sola célula que, a su vez, se puede utilizar para un inmunofenotipado robusto.
