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Isolation of Lamina Propria Mononuclear Cells from Murine Colon Using Collagenase E
JoVE Journal
免疫学と感染
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JoVE Journal 免疫学と感染
Isolation of Lamina Propria Mononuclear Cells from Murine Colon Using Collagenase E

Isolation of Lamina Propria Mononuclear Cells from Murine Colon Using Collagenase E

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09:48 min

September 26, 2019

DOI:

09:48 min
September 26, 2019

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概要

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The goal of this protocol is to isolate mononuclear cells that reside in the lamina propria of the colon by enzymatic digestion of the tissue using collagenase. This protocol allows for the efficient isolation of mononuclear cells resulting in a single cell suspension which in turn can be used for robust immunophenotyping.

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