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Kwantificering van monocyten Chemotactische activiteit in vivo en karakterisering van bloed monocyten afgeleide macrofagen
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages
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Kwantificering van monocyten Chemotactische activiteit in vivo en karakterisering van bloed monocyten afgeleide macrofagen

Nederlands

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9,341 Views

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09:57 min

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August 12, 2019

DOI:

10.3791/59706-v

DOI:
10.3791/59706-v

•

09:57 min
August 12, 2019

•

3 Views
, ,
1Department of Internal Medicine, Section on Molecular Medicine,Wake Forest School of Medicine, 2Department of Biochemistry,Wake Forest School of Medicine

Summary

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Hier presenteren we een protocol om de Chemotactische activiteit van bloed monocyten in muizen modellen te kwantificeren, om de effecten van nutritionele, farmacologische en genetische interventies op bloed monocyten te beoordelen en om de in het bloed geafgeleide macrofagen te karakteriseren in Muismodellen met behulp van monocyte-chemoaantretant proteïne-1 (MCP-1)-geladen in de kelder membraan-afgeleide gel stekkers.

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Ahn, Y. J., Wang, L., Asmis, R. Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages. J. Vis. Exp. (150), e59706, doi:10.3791/59706 (2019).

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