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Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages
Chapters
- 00:04Title
- 00:47Preparation of the Monocyte Chemoattractant Protein (MCP)-1-loaded Growth Factor-reduced Basement Membrane-derived Solution
- 01:40Basement Membrane-derived Solution Injection and Gel Plug Harvest
- 03:29Basement Membrane-derived Gel Plug Digestion
- 04:43Single-cell Western Blot (scWB) Analysis
- 06:38scWB Chip Stripping
- 07:35Results: Representative Basement Membrane-derived Gel Plug Cell Recruitment Analyses
- 09:22Conclusion
Here we present a protocol to quantify the chemotactic activity of blood monocytes in mouse models, to assess the effects of nutritional, pharmacological and genetic interventions on blood monocyte and to characterize the blood monocytes derived macrophages in mouse models using monocyte-chemoattractant protein-1 (MCP-1)-loaded basement membrane-derived gel plugs.
