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Toepassing van Biolayer interferometrie (BLI) voor het bestuderen van eiwit-eiwit interacties in transcriptie
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Biochemistry
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JoVE Journal Biochemistry
Application of Biolayer Interferometry (BLI) for Studying Protein-Protein Interactions in Transcription
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Toepassing van Biolayer interferometrie (BLI) voor het bestuderen van eiwit-eiwit interacties in transcriptie

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13,091 Views

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07:18 min

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July 26, 2019

DOI:

10.3791/59687-v

DOI:
10.3791/59687-v

•

07:18 min
July 26, 2019

•

12 Views
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1Department of Pharmacology, Robert Wood Johnson Medical School,Rutgers University, 2Graduate Program in Physiology and Integrative Biology, School of Graduate Studies,Rutgers University, 3Department of Plant Biology, School of Environmental and Biological,Rutgers University

Summary

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Interacties van transcriptiefactoren (TFs) met de RNA-polymerase worden meestal bestudeerd met behulp van keuze assays. We passen een Biolayer interferometrie (BLI) technologie toe om de interactie van GrgA met de chlamydiale RNA-polymerase te karakteriseren. Vergeleken met keuze assays, bli detecteert real-time associatie en dissociatie, biedt een hogere gevoeligheid, en is zeer kwantitatief.

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Cite this Article

Desai, M., Di, R., Fan, H. Application of Biolayer Interferometry (BLI) for Studying Protein-Protein Interactions in Transcription. J. Vis. Exp. (149), e59687, doi:10.3791/59687 (2019).

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