/
/
Application of Biolayer Interferometry (BLI) for Studying Protein-Protein Interactions in Transcription
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Application of Biolayer Interferometry (BLI) for Studying Protein-Protein Interactions in Transcription
13,074 Views
•
07:18 min
•
July 26, 2019
DOI:
Summary
Automatically generated
Interactions of transcription factors (TFs) with the RNA polymerase are usually studied using pulldown assays. We apply a Biolayer Interferometry (BLI) technology to characterize the interaction of GrgA with the chlamydial RNA polymerase. Compared to pulldown assays, BLI detects real-time association and dissociation, offers higher sensitivity, and is highly quantitative.
Read Article
Cite this Article
Desai, M., Di, R., Fan, H. Application of Biolayer Interferometry (BLI) for Studying Protein-Protein Interactions in Transcription. J. Vis. Exp. (149), e59687, doi:10.3791/59687 (2019).
Copy