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Endotel hücrelerinden RNA yalıtım için Ribosome affinity arıtma (TRAP) çevirisi In vivo
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08:53 min
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May 25, 2019
DOI:
1Blood Research Institute,Blood Center of Wisconsin, 2Department of Medicine,Medical College of Wisconsin, 3Department of Surgery,The University of Alabama at Birmingham, 4Department of Biomedical Engineering,The University of Alabama at Birmingham, 5GBS Program, Graduate School,The University of Alabama at Birmingham
Summary
Automatically generated
Eğer RNA arıtma ile birlikte ribozomlarda içinde gelişmiş yeşil floresan proteinin (EGFP) EC özgü genetik etiketi ile doğrudan fare beyin, akciğer ve kalp dokularda vasküler endotel hücrelerden (ECS) ribosome bağlı mRNA arındırmak için bir yaklaşım sunuyoruz .
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Cite this Article
Moran, P., Guo, Y., Yuan, R., Barnekow, N., Palmer, J., Beck, A., Ren, B. Translating Ribosome Affinity Purification (TRAP) for RNA Isolation from Endothelial Cells In Vivo. J. Vis. Exp. (147), e59624, doi:10.3791/59624 (2019).
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