ジャーナル
/
生化学
/
Translating Ribosome Affinity Purification (TRAP) for RNA Isolation from Endothelial Cells In Vivo
JoVE Journal
生化学
Author Produced
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 生化学
Translating Ribosome Affinity Purification (TRAP) for RNA Isolation from Endothelial Cells In Vivo

Translating Ribosome Affinity Purification (TRAP) for RNA Isolation from Endothelial Cells In Vivo

11,478 Views

•

08:53 min

•

May 25, 2019

DOI:

10.3791/59624-v

DOI:
10.3791/59624-v

•

08:53 min
May 25, 2019

•

13 Views
, , , , , ,
1Blood Research Institute,Blood Center of Wisconsin, 2医学部,Medical College of Wisconsin, 3外科,The University of Alabama at Birmingham, 4Department of Biomedical Engineering,The University of Alabama at Birmingham, 5GBS Program, Graduate School,The University of Alabama at Birmingham

概要

Automatically generated

We present an approach to purify ribosome-bound mRNA from vascular endothelial cells (ECs) directly in mouse brain, lung and heart tissues via EC-specific genetic tag of enhanced green fluorescence protein (EGFP)in ribosomes in combination with RNA purification.

Explore More Videos

RNA IsolationEndothelial CellsRibosome Affinity Purification (TRAP)Transgenic MiceGFP-tagged RibosomesTranscriptome AnalysisAngiogenesisエピジェネティクスTranscription RegulationGenotypingRNA SequencingRT-qPCR

Read Article

Cite this Article

Moran, P., Guo, Y., Yuan, R., Barnekow, N., Palmer, J., Beck, A., Ren, B. Translating Ribosome Affinity Purification (TRAP) for RNA Isolation from Endothelial Cells In Vivo. J. Vis. Exp. (147), e59624, doi:10.3791/59624 (2019).

Download .ris file

Copy

Share Video

.

Copy