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Translating Ribosome Affinity Purification (TRAP) for RNA Isolation from Endothelial Cells In Vivo
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JoVE Journal 生化学
Translating Ribosome Affinity Purification (TRAP) for RNA Isolation from Endothelial Cells In Vivo

Translating Ribosome Affinity Purification (TRAP) for RNA Isolation from Endothelial Cells In Vivo

DOI:
10.3791/59624-v

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08:53 min

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May 25, 2019

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, , , , , ,
1Blood Research Institute,Blood Center of Wisconsin, 2医学部,Medical College of Wisconsin, 3外科,The University of Alabama at Birmingham, 4Department of Biomedical Engineering,The University of Alabama at Birmingham, 5GBS Program, Graduate School,The University of Alabama at Birmingham

章

  • 00:00標題
  • 02:34Methods
  • 07:06結果
  • 07:39Conclusion

概要

自動翻訳

自動翻訳

We present an approach to purify ribosome-bound mRNA from vascular endothelial cells (ECs) directly in mouse brain, lung and heart tissues via EC-specific genetic tag of enhanced green fluorescence protein (EGFP)in ribosomes in combination with RNA purification.

タグ

RNA IsolationEndothelial CellsRibosome Affinity Purification (TRAP)Transgenic MiceGFP-tagged RibosomesTranscriptome AnalysisAngiogenesisエピジェネティクスTranscription RegulationGenotypingRNA SequencingRT-qPCR

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