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Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells
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JoVE Journal 免疫学と感染
Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells

Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells

8,942 Views

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08:09 min

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June 19, 2019

DOI:

10.3791/59617-v

DOI:
10.3791/59617-v

•

08:09 min
June 19, 2019

•

9 Views
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1Division of Inflammation Biology,La Jolla Institute for Immunology, 2Flow Cytometry Core Facility,La Jolla Institute for Immunology

概要

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Here, we present a protocol to process fresh bone marrow (BM) isolated from mouse or human for high-dimensional mass cytometry (Cytometry by Time-Of-Flight, CyTOF) analysis of neutrophil-lineage cells.

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Cite this Article

Zhu, Y. P., Padgett, L., Dinh, H. Q., Marcovecchio, P., Wu, R., Hinz, D., Kim, C., Hedrick, C. C. Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells. J. Vis. Exp. (148), e59617, doi:10.3791/59617 (2019).

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