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단일 T 세포또는 면역 학적 단세포 힘 분광학을 위한 단입자를 가진 원자력 현미경 캔틸레버의 기능화
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Immunology and Infection
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JoVE Journal Immunology and Infection
Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy
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단일 T 세포또는 면역 학적 단세포 힘 분광학을 위한 단입자를 가진 원자력 현미경 캔틸레버의 기능화

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10:06 min

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July 10, 2019

DOI:

10.3791/59609-v

DOI:
10.3791/59609-v

•

10:06 min
July 10, 2019

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1State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine,Peking Union Medical College, 2Institute for Immunology, School of Medicine,Tsinghua University

Tags

Atomic Force MicroscopeCantilever FunctionalizationSingle T CellSingle ParticleImmunological Force SpectroscopyDendritic Cell-T Cell InteractionCell-surface InteractionBiocompatible GlueIL-2Sample PreparationCalibrationLiving Cell Environment

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Chen, J., Xu, Y., Shi, Y., Xia, T. Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy. J. Vis. Exp. (149), e59609, doi:10.3791/59609 (2019).

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