JoVE Journal
Immunology and Infection
Immunology and Infection
需要订阅 JoVE 才能查看此 内容. 登录或开始免费试用。
章节
总结概括
We present a protocol to functionalize atomic force microscope (AFM) cantilevers with a single T cell and bead particle for immunological studies. Procedures to probe single-pair T cell-dendritic cell binding by AFM and to monitor the real-time cellular response of macrophages to a single solid particle by AFM with fluorescence imaging are shown.