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三维流状入侵检测中的全人肿瘤基基质
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal Cancer Research
Fully Human Tumor-based Matrix in Three-dimensional Spheroid Invasion Assay
DOI:

08:15 min

May 07, 2019

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Chapters

  • 00:04Title
  • 01:10Multicellular Tumor Spheroid Generation and 3D Spheroid Invasion Assay
  • 02:53Image Segmentation With Ilastik
  • 04:39Area Analysis with Fiji ImageJ
  • 06:23Results: Representative Cell Movement in a 3D Invasion Model
  • 07:36Conclusion

Summary

Automatic Translation

肿瘤微环境是癌症生长和侵袭的重要组成部分。为了模仿癌症的进展, 需要一个与生物学相关的人类基质。该方案介绍了应用人平滑肌瘤基基质进行体外三维球体入侵检测的改进。该协议还介绍了基于计算机的细胞入侵分析。

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