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Bacterial Cell Culture at the Single-cell Level Inside Giant Vesicles
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Chapters
- 00:04Title
- 00:31Lipid-containing Oil Solution and Small Vesicle (SV) Preparation
- 01:54Bacterial Cell Pre-culture
- 02:45Giant Vesicle (GV) and Water-in-Oil (W/O) Bacterial Cell-containing Droplet Formation
- 04:11Handmade Chamber and Supported Bilayer Membrane Preparation
- 04:54GV Immobilization and Microscopic Bacterial Growth Observation
- 06:04Results: Representative GV Containing Bacteria and Bacterial Growth Evaluation
- 06:44Conclusion
We demonstrate single-cell culture of bacteria inside giant vesicles (GVs). GVs containing bacterial cells were prepared by the droplet transfer method and were immobilized on a supported membrane on a glass substrate for direct observation of bacterial growth. This approach may also be adaptable to other cells.
