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从鸡蛋黄中分离脂蛋白颗粒,研究细菌病原体脂肪酸并入膜磷脂
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Biochemistry
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JoVE Journal Biochemistry
Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids
Please note that all translations are automatically generated. Click here for the English version.

从鸡蛋黄中分离脂蛋白颗粒,研究细菌病原体脂肪酸并入膜磷脂

English

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9,468 Views

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11:59 min

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May 15, 2019

DOI:

10.3791/59538-v

DOI:
10.3791/59538-v

•

11:59 min
May 15, 2019

•

4 Views
, ,
1Department of Microbiology and Molecular Genetics,Michigan State University, 2Department of Physiology,Michigan State University

Summary

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该方法为研究将来自复杂宿主来源的外源脂肪酸结合到细菌膜中提供了一个框架,特别是金黄色葡萄球菌。为此,介绍了从鸡蛋蛋黄中浓缩脂蛋白颗粒的方案,以及利用质谱法对细菌磷脂进行随后脂肪酸分析的介绍。

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Cite this Article

Delekta, P. C., Lydic, T. A., Hammer, N. D. Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids. J. Vis. Exp. (147), e59538, doi:10.3791/59538 (2019).

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