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Immunology and Infection
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在自抗性混合奇美体模型中, 光激活询问个体自反应萌发中心
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Interrogating Individual Autoreactive Germinal Centers by Photoactivation in a Mixed Chimeric Model of Autoimmunity
Please note that all translations are automatically generated.
Click here for the English version.
在自抗性混合奇美体模型中, 光激活询问个体自反应萌发中心
DOI:
10.3791/59397-v
•
11:12 min
•
April 11, 2019
•
Thomas R. Wittenborn
,
Cecilia Hagert
,
Søren E Degn
1
Department of Biomedicine
,
Aarhus University
Chapters
00:04
Title
01:16
Bone Harvest
02:26
Bone Marrow Cell Extraction
03:16
Bone Marrow Recipient Reconstitution
04:48
Popliteal Lymph Node Labelling and Harvest
06:54
Single Splenic Germinal Center Identification
08:43
Results: Representative Germinal Center Photoactivation
10:19
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该方案描述了混合小鼠骨髓嵌合体与自发自身免疫性萌发中心的生成, 其中自反应淋巴细胞携带光可活性的绿色荧光蛋白 (PA-GFP) 记者。这提供了将细胞在组织中的位置与下游分子和功能分析联系起来的能力。
Tags
Autoimmunity
Autoreactive Germinal Centers
Mixed Chimeric Model
Photoactivation
Bone Marrow Chimera
Immunology
Immuno-oncology
Stem Cell Research
Bone Marrow Extraction
Bone Marrow Reconstitution
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