Journal
/
Genética
/
Sequencing of mRNA from Whole Blood using Nanopore Sequencing
JoVE Journal
Genética
This content is Free Access.
JoVE Journal Genética
Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

13,041 Views

•

11:26 min

•

June 03, 2019

DOI:

10.3791/59377-v

DOI:
10.3791/59377-v

•

11:26 min
June 03, 2019

•

11 Views
, , ,
1Institute of Molecular Virology and Cell Biology,Friedrich-Loeffler-Institut, 2Institute of Novel and Emerging Infectious Diseases,Friedrich-Loeffler-Institut, 3Department of Animal Science,Njala University

Summary

Automatically generated

Nanopore sequencing is a novel technology that allows cost-effective sequencing in remote locations and resource-poor settings. Here, we present a protocol for sequencing of mRNAs from whole blood that is compatible with such conditions.

Explore More Videos

MRNA SequencingNanopore SequencingThird-generation SequencingField LaboratoriesEbola VirusTouchdown PCRCDNA AmplificationMagnetic Bead PurificationEthanol WashLibrary Preparation

Read Article

Cite this Article

Mueller, A., Fischer, K., Suluku, R., Hoenen, T. Sequencing of mRNA from Whole Blood using Nanopore Sequencing. J. Vis. Exp. (148), e59377, doi:10.3791/59377 (2019).

Download .ris file

Copy

Share Video

.

Copy