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Post-differentiation Replating of Human Pluripotent Stem Cell-derived Neurons for High-content Screening of Neuritogenesis and Synapse Maturation
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Neurociência
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JoVE Journal Neurociência
Post-differentiation Replating of Human Pluripotent Stem Cell-derived Neurons for High-content Screening of Neuritogenesis and Synapse Maturation

Post-differentiation Replating of Human Pluripotent Stem Cell-derived Neurons for High-content Screening of Neuritogenesis and Synapse Maturation

DOI:
10.3791/59305-v

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00:06 min

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August 28, 2019

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, , ,
1Division of Biological Sciences,University of California, San Diego, 2Sanford Consortium for Regenerative Medicine

Capítulos

  • 00:05Título
  • 01:22Replating Differentiated Neurons
  • 04:22Cell Viability Assays After Replating
  • 04:44Results: Replating of Differentiated iPSC-Derived Neurons
  • 06:09Conclusion

Summary

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Tadução automática

This protocol describes a detailed procedure for resuspending and culturing human stem cell derived neurons that were previously differentiated from neural progenitors in vitro for multiple weeks. The procedure facilitates imaging-based assays of neurites, synapses, and late-expressing neuronal markers in a format compatible with light microscopy and high-content screening.

Tags

Human Pluripotent Stem CellsNeuronsHigh-content ScreeningNeuritogenesisSynapse MaturationReplatingProteaseCell CultureDissociationTitrationCentrifugation

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