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Microfocus X-ray CT (microCT) Imaging of Actinia equina (Cnidaria), Harmothoe sp. (Annelida), and Xenoturbella japonica (Xenacoelomorpha)
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Microfocus X-ray CT (microCT) Imaging of Actinia equina (Cnidaria), Harmothoe sp. (Annelida), and Xenoturbella japonica (Xenacoelomorpha)

Microfocus X-ray CT (microCT) Imaging of Actinia equina (Cnidaria), Harmothoe sp. (Annelida), and Xenoturbella japonica (Xenacoelomorpha)

DOI:
10.3791/59161-v

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08:09 min

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August 06, 2019

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, , ,
1Mammalian Genetics Laboratory,National Institute of Genetics, 2Misaki Marine Biological Station,The University of Tokyo, 3Shimoda Marine Research Center,University of Tsukuba

Chapters

  • 00:04Title
  • 00:38Stage Mounting
  • 04:17MicroCT Scanning
  • 06:24Results: Scanned and Reconstructed Images of Marine Invertebrates
  • 07:24Conclusion

Summary

Automatic Translation

Automatic Translation

Here, protocols for performing microfocus X-ray computed tomography (microCT) imaging of three marine invertebrate animals are explained in detail. This study describes steps such as sample fixation, staining, mounting, scanning, image reconstruction, and data analyses. Suggestions on how the protocol can be adjusted for different samples are also provided.

Tags

Keywords: MicroCTActinia EquinaHarmothoe Sp.Xenoturbella JaponicaMarine InvertebratesSample MountingAgaroseStainingSample Preparation

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