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Microfocus X-ray CT (microCT) Imaging of Actinia equina (Cnidaria), Harmothoe sp. (Annelida), and Xenoturbella japonica (Xenacoelomorpha)
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JoVE Revista Medio ambiente
Microfocus X-ray CT (microCT) Imaging of Actinia equina (Cnidaria), Harmothoe sp. (Annelida), and Xenoturbella japonica (Xenacoelomorpha)

Microfocus X-ray CT (microCT) Imaging of Actinia equina (Cnidaria), Harmothoe sp. (Annelida), and Xenoturbella japonica (Xenacoelomorpha)

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08:09 min

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August 06, 2019

DOI:

10.3791/59161-v

DOI:
10.3791/59161-v

•

08:09 min
August 06, 2019

•

3 Views
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1Mammalian Genetics Laboratory,National Institute of Genetics, 2Misaki Marine Biological Station,The University of Tokyo, 3Shimoda Marine Research Center,University of Tsukuba

Summary

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Here, protocols for performing microfocus X-ray computed tomography (microCT) imaging of three marine invertebrate animals are explained in detail. This study describes steps such as sample fixation, staining, mounting, scanning, image reconstruction, and data analyses. Suggestions on how the protocol can be adjusted for different samples are also provided.

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MicroCTActinia EquinaHarmothoe Sp.Xenoturbella JaponicaMarine InvertebratesSample MountingAgaroseStainingSample Preparation

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Cite this Article

Maeno, A., Kohtsuka, H., Takatani, K., Nakano, H. Microfocus X-ray CT (microCT) Imaging of Actinia equina (Cnidaria), Harmothoe sp. (Annelida), and Xenoturbella japonica (Xenacoelomorpha). J. Vis. Exp. (150), e59161, doi:10.3791/59161 (2019).

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