This protocol enables visualization of gene expression in embryonic Astyanax cavefish. This approach has been developed with the goal of maximizing gene expression signal, while minimizing non-specific background staining.
Luc, H., Sears, C., Raczka, A., Gross, J. B. Wholemount In Situ Hybridization for Astyanax Embryos. J. Vis. Exp. (145), e59114, doi:10.3791/59114 (2019).