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マウス頭蓋組織および非除電骨の組織調製と免疫染色
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Developmental Biology
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JoVE Journal Developmental Biology
Tissue Preparation and Immunostaining of Mouse Craniofacial Tissues and Undecalcified Bone
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マウス頭蓋組織および非除電骨の組織調製と免疫染色

日本語

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12,056 Views

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10:03 min

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May 10, 2019

DOI:

10.3791/59113-v

DOI:
10.3791/59113-v

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10:03 min
May 10, 2019

•

7 Views
, ,
1The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine of Ministry of Education, School and Hospital of Stomatology,Wuhan University, 2Department of Biologic and Materials Sciences, School of Dentistry,University of Michigan

Summary

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ここでは、マウス頭蓋組織を例に免疫染色することにより、頭蓋顔面形態/病因の間のタンパク質レベルを検出し、定量するための詳細なプロトコルを提示する。また、免疫染色のための若いマウスからの非デカル化された硬質組織の調製および凍結切除の方法について説明する。

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Cite this Article

Yang, J., Pan, H., Mishina, Y. Tissue Preparation and Immunostaining of Mouse Craniofacial Tissues and Undecalcified Bone. J. Vis. Exp. (147), e59113, doi:10.3791/59113 (2019).

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