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Síntese e Bioconjugation dos reagentes Thiol-reativa para a criação de Site-seletivamente modificado Immunoconjugates
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Chemistry
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JoVE Journal Chemistry
Synthesis and Bioconjugation of Thiol-Reactive Reagents for the Creation of Site-Selectively Modified Immunoconjugates

Síntese e Bioconjugation dos reagentes Thiol-reativa para a criação de Site-seletivamente modificado Immunoconjugates

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9,076 Views

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08:47 min

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March 06, 2019

DOI:

10.3791/59063-v

DOI:
10.3791/59063-v

•

08:47 min
March 06, 2019

•

2 Views
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1Department of Chemistry,Hunter College of the City University of New York, 2Ph.D. Program in Chemistry,Graduate Center of the City University of New York, 3Department of Radiology,Memorial Sloan Kettering Cancer Center, 4Department of Radiology,Weill Cornell Medical College

Summary

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Neste protocolo, descreveremos a síntese de vagens, phenyoxadiazolyl metil sulfona-baseado reagente para a ligação local-seletivo de cargas para os tióis de biomoléculas, especialmente de anticorpos. Além disso, descreveremos a síntese e caracterização de um quelante bifuncional PODS-rolamento e sua conjugação com um anticorpo de modelo.

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Davydova, M., Dewaele Le Roi, G., Adumeau, P., Zeglis, B. M. Synthesis and Bioconjugation of Thiol-Reactive Reagents for the Creation of Site-Selectively Modified Immunoconjugates. J. Vis. Exp. (145), e59063, doi:10.3791/59063 (2019).

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