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Synthesis and Bioconjugation of Thiol-Reactive Reagents for the Creation of Site-Selectively Modified Immunoconjugates
JoVE Journal
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Synthesis and Bioconjugation of Thiol-Reactive Reagents for the Creation of Site-Selectively Modified Immunoconjugates

Synthesis and Bioconjugation of Thiol-Reactive Reagents for the Creation of Site-Selectively Modified Immunoconjugates

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08:47 min

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March 06, 2019

DOI:

10.3791/59063-v

DOI:
10.3791/59063-v

•

08:47 min
March 06, 2019

•

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1Kimya Bölümü,Hunter College of the City University of New York, 2Ph.D. Program in Chemistry,Graduate Center of the City University of New York, 3Department of Radiology,Memorial Sloan Kettering Kanser Merkezi, 4Department of Radiology,Weill Cornell Medical College

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In this protocol, we will describe the synthesis of PODS, a phenyoxadiazolyl methyl sulfone-based reagent for the site-selective attachment of cargos to the thiols of biomolecules, particularly antibodies. In addition, we will describe the synthesis and characterization of a PODS-bearing bifunctional chelator and its conjugation to a model antibody.

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Davydova, M., Dewaele Le Roi, G., Adumeau, P., Zeglis, B. M. Synthesis and Bioconjugation of Thiol-Reactive Reagents for the Creation of Site-Selectively Modified Immunoconjugates. J. Vis. Exp. (145), e59063, doi:10.3791/59063 (2019).

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