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硫醇反应试剂的合成与生物共轭作用--用于原位选择性修饰免疫共轭的生成

JoVE Journal
Chemistry

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JoVE Journal Chemistry

Synthesis and Bioconjugation of Thiol-Reactive Reagents for the Creation of Site-Selectively Modified Immunoconjugates

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硫醇反应试剂的合成与生物共轭作用--用于原位选择性修饰免疫共轭的生成

9,076 Views

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08:47 min

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March 06, 2019

DOI:

10.3791/59063-v

DOI:

10.3791/59063-v

•

08:47 min

March 06, 2019

•

2 Views

Maria Davydova¹, Guillaume Dewaele Le Roi^1,2, Pierre Adumeau¹, Brian M. Zeglis^1,2,3,4

¹Department of Chemistry,Hunter College of the City University of New York, ²Ph.D. Program in Chemistry,Graduate Center of the City University of New York, ³Department of Radiology,Memorial Sloan Kettering Cancer Center, ⁴Department of Radiology,Weill Cornell Medical College

Summary

在本议定书中, 我们将描述 pods 的合成, 这是一种基于苯氧二唑基甲基磺酸试剂的合成, 用于将货物选择性地附着在生物分子的硫醇中, 特别是抗体中。此外, 我们将描述含有 pod 的双官能螯合剂的合成和表征及其与模型抗体的结合。

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