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A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
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JoVE 杂志 遗传学
A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

DOI:
10.3791/59055-v

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07:10 min

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February 19, 2019

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, , ,
1Department of Biology,Georgetown University

Chapters

  • 00:04Title
  • 00:38Bacterial Inoculum Preparation and Infection
  • 02:17Organ Harvest and Tissue Processing
  • 03:20Laser Scanning Confocal Microscopy and Image Processing
  • 04:52Results: Representative Localization of nuc-sGFP in the Staphylococcal Abscess Microenvironment
  • 06:22Conclusion

Summary

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Described here is a method for analyzing bacterial gene expression in animal tissues at a cellular level. This method provides a resource for studying the phenotypic diversity occurring within a bacterial population in response to the tissue environment during an infection.

Tags

Bacterial Gene RegulationFluorescence-based MethodBacterial Gene ExpressionS. AureusHemolytic PhenotypeBacterial PhysiologyAntivirulenceAntibacterial CompoundsCryosectioningDAPI Staining

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