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En høyt innhold analysen for overvåking AMPA reseptor menneskehandel
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
A High-content Assay for Monitoring AMPA Receptor Trafficking
DOI:

10:34 min

January 28, 2019

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Chapters

  • 00:04Title
  • 01:17Preparation of Primary Hippocampal Neurons and Measuring AMPA Receptor Trafficking in Response to DHPG
  • 02:26Immunolabelling (performed in Laminar Flow Hood)
  • 04:09Labeling Surface Receptors
  • 05:07Labeling the Internalized Receptor Population
  • 06:18Imaging and Analysis
  • 08:07Results: Analysis of AMPA Receptor Trafficking Using the High-content Assay
  • 09:10Conclusion

Summary

Automatic Translation

Neuronal excitability kan være modulert gjennom en dynamisk prosess endo- og exocytosis av eksitatoriske ionotropic glutamat reseptorer. Beskrevet her er tilgjengelig, høyt innhold analysen for kvantifisering overflate og interne reseptor befolkningen bassenger.

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