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一种用于监测 ampa 受体贩运的高含量检测方法
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
A High-content Assay for Monitoring AMPA Receptor Trafficking
DOI:

10:34 min

January 28, 2019

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Chapters

  • 00:04Title
  • 01:17Preparation of Primary Hippocampal Neurons and Measuring AMPA Receptor Trafficking in Response to DHPG
  • 02:26Immunolabelling (performed in Laminar Flow Hood)
  • 04:09Labeling Surface Receptors
  • 05:07Labeling the Internalized Receptor Population
  • 06:18Imaging and Analysis
  • 08:07Results: Analysis of AMPA Receptor Trafficking Using the High-content Assay
  • 09:10Conclusion

Summary

Automatic Translation

神经兴奋性可以通过兴奋性电离谷氨酸受体的内和外渗的动态过程来调节。这里描述的是一个可访问的, 高含量的检测定量表面和内部受体群体池。

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