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Neuroscience
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一种用于监测 ampa 受体贩运的高含量检测方法
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
A High-content Assay for Monitoring AMPA Receptor Trafficking
Please note that all translations are automatically generated.
Click here for the English version.
一种用于监测 ampa 受体贩运的高含量检测方法
DOI:
10.3791/59048-v
•
10:34 min
•
January 28, 2019
•
Mohammad A. Ghane*
1
,
Dina W. Yakout*
1
,
Angela M. Mabb
1
Neuroscience Institute
,
Georgia State University
Chapters
00:04
Title
01:17
Preparation of Primary Hippocampal Neurons and Measuring AMPA Receptor Trafficking in Response to DHPG
02:26
Immunolabelling (performed in Laminar Flow Hood)
04:09
Labeling Surface Receptors
05:07
Labeling the Internalized Receptor Population
06:18
Imaging and Analysis
08:07
Results: Analysis of AMPA Receptor Trafficking Using the High-content Assay
09:10
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
神经兴奋性可以通过兴奋性电离谷氨酸受体的内和外渗的动态过程来调节。这里描述的是一个可访问的, 高含量的检测定量表面和内部受体群体池。
Tags
AMPA Receptor Trafficking
High-content Assay
Primary Neuronal Culture
Surface And Intracellular Receptor Pools
Receptor Trafficking Profiles
Synaptic Loss
Alzheimer’s Disease
TTX Treatment
Anti-GluA1/GluA2 Antibody
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