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基于逆电子需求函数-alder 反应的隐形放射免疫治疗
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Chemistry
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JoVE Journal Chemistry
Pretargeted Radioimmunotherapy Based on the Inverse Electron Demand Diels-Alder Reaction
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基于逆电子需求函数-alder 反应的隐形放射免疫治疗

DOI:
10.3791/59041-v

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09:44 min

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January 29, 2019

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, ,
1Department of Chemistry,Hunter College of the City University of New York, 2Ph.D. Program in Chemistry,Graduate Center of the City University of New York, 3Department of Radiology,Memorial Sloan Kettering Cancer Center, 4Department of Radiology,Weill Cornell Medical College

Chapters

  • 00:04Title
  • 00:41The Preparation of huA33-TCO
  • 03:54177Lu Radiolabeling of Tz-PEG7-DOTA
  • 05:10In vivo Studies
  • 07:08Results: Longitudinal Therapy Study of Mice Bearing Subcutaneous SW1222 Tumors
  • 09:00Conclusion

Summary

Automatic Translation

Automatic Translation

该协议描述了反式环磷 (tco) 修饰抗体和177个lu-tazine (tz) 辐射寡核苷酸的合成和表征, 用于预定目标放射治疗 (prit)。此外, 它还详细介绍了这两种结构在结直肠癌小鼠模型中的应用, 用于体内生物分布和纵向治疗研究。

Tags

Pretargeted RadioimmunotherapyPRITInverse Electron Demand Diels-Alder ReactionColorectal Cancer XenograftAntibody ConjugationTCO-NHSHuA33 AntibodySize Exclusion ChromatographyUV-vis Spectrophotometry

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