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Mit In-vitro- und In der Zelle Form zu untersuchen, kleines Molekül induzierte Pre-mRNA Strukturveränderungen
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Genetics
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JoVE Journal Genetics
Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes
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Mit In-vitro- und In der Zelle Form zu untersuchen, kleines Molekül induzierte Pre-mRNA Strukturveränderungen

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8,117 Views

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11:58 min

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January 30, 2019

DOI:

10.3791/59021-v

DOI:
10.3791/59021-v

•

11:58 min
January 30, 2019

•

11 Views
, ,
1Calibr,The Scripps Research Institute, 2Department of Integrative Structural and Computational Biology,The Scripps Research Institute

Summary

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Detaillierte Protokolle für beide in vitro und in-Cell selektive 2'-Hydroxyl-Acylation von Primer Extension (Form) Experimente zur Bestimmung der sekundären Struktur der Pre-mRNA-Sequenzen von Interesse im Beisein einer RNA-targeting niedermolekularer analysiert werden in diesem Artikel vorgestellt.

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Wang, J., Hammond, J., Johnson, K. A. Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes. J. Vis. Exp. (143), e59021, doi:10.3791/59021 (2019).

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