Rivista
/
Genetica
/
Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes
JoVE Journal
Genetica
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Genetica
Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes

8,117 Views

•

11:58 min

•

January 30, 2019

DOI:

10.3791/59021-v

DOI:
10.3791/59021-v

•

11:58 min
January 30, 2019

•

11 Views
, ,
1Calibr,The Scripps Research Institute, 2Department of Integrative Structural and Computational Biology,The Scripps Research Institute

Summary

Automatically generated

Detailed protocols for both in vitro and in-cell selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE) experiments to determine the secondary structure of pre-mRNA sequences of interest in the presence of an RNA-targeting small molecule are presented in this article.

Explore More Videos

In Vitro SHAPEIn-cell SHAPERNA Secondary StructureSmall MoleculePre-mRNAReverse TranscriptionRadioactive LabelingAcrylamide GelPassive Elution

Read Article

Cite this Article

Wang, J., Hammond, J., Johnson, K. A. Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes. J. Vis. Exp. (143), e59021, doi:10.3791/59021 (2019).

Download .ris file

Copy

Share Video

.

Copy