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Cultura de células primárias de neurônios Gabaérgicos ou glutamatérgicos purificados estabelecidos através da triagem de células ativadas por fluorescência
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Primary Cell Culture of Purified GABAergic or Glutamatergic Neurons Established through Fluorescence-activated Cell Sorting

Cultura de células primárias de neurônios Gabaérgicos ou glutamatérgicos purificados estabelecidos através da triagem de células ativadas por fluorescência

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10,831 Views

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10:57 min

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June 06, 2019

DOI:

10.3791/58974-v

DOI:
10.3791/58974-v

•

10:57 min
June 06, 2019

•

5 Views
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1Institut für Integrative Neuroanatomie, Charité,Universitätsmedizin Berlin, 2Department for Cytometry and Cellsorting, German Rheumatism Research Center,Leibniz Institute, 3Departments of Genetic and Behavioral Neuroscience,Graduate School of Medicine, Gunma University

Summary

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Este protocolo descreve um método baseado classificação da pilha para a purificação e a cultura de neurônios gabaergic ou glutamatérgico fluorescentes do neocórtex e do hipocampo de ratos ou de ratos pós-natal.

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Turko, P., Groberman, K., Kaiser, T., Yanagawa, Y., Vida, I. Primary Cell Culture of Purified GABAergic or Glutamatergic Neurons Established through Fluorescence-activated Cell Sorting. J. Vis. Exp. (148), e58974, doi:10.3791/58974 (2019).

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