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Levantando o Tetra mexicano Astyanax mexicanus para análise dos fenótipos Post-larval e imuno-histoquímica toda a montagem
JoVE Journal
Genetics
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JoVE Journal Genetics
Raising the Mexican Tetra Astyanax mexicanus for Analysis of Post-larval Phenotypes and Whole-mount Immunohistochemistry

Levantando o Tetra mexicano Astyanax mexicanus para análise dos fenótipos Post-larval e imuno-histoquímica toda a montagem

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8,328 Views

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06:42 min

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December 28, 2018

DOI:

10.3791/58972-v

DOI:
10.3791/58972-v

•

06:42 min
December 28, 2018

•

4 Views
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1Harvard Medical School

Summary

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Neste protocolo, demonstramos como Astyanax mexicanus adultos se reproduzem, levantar as larvas e executar toda a montagem imuno-histoquímica na pós larval peixe para comparar os fenótipos de superfície e caverna morfotipos.

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Riddle, M., Martineau, B., Peavey, M., Tabin, C. Raising the Mexican Tetra Astyanax mexicanus for Analysis of Post-larval Phenotypes and Whole-mount Immunohistochemistry. J. Vis. Exp. (142), e58972, doi:10.3791/58972 (2018).

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