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Raising the Mexican Tetra Astyanax mexicanus for Analysis of Post-larval Phenotypes and Whole-mount Immunohistochemistry
JoVE Journal
Genetics
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JoVE Journal Genetics
Raising the Mexican Tetra Astyanax mexicanus for Analysis of Post-larval Phenotypes and Whole-mount Immunohistochemistry

Raising the Mexican Tetra Astyanax mexicanus for Analysis of Post-larval Phenotypes and Whole-mount Immunohistochemistry

DOI:
10.3791/58972-v

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06:42 min

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December 28, 2018

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, , ,
1Harvard Medical School

Chapters

  • 00:04Title
  • 00:38Breeding
  • 01:20Hatching Fertilized Eggs
  • 02:16Transfer of Hatched Larvae to Nursery Containers
  • 02:45Preparation of Rotifer-based Fish Food and Feeding of Post-Larval Fish
  • 03:19Whole-Mount Immunohistochemistry of Post-Larval Fish
  • 05:11Results: Surface Fish Produce Greatest Number of Larvae per Spawn
  • 05:56Conclusion

Summary

Automatic Translation

Automatic Translation

In this protocol, we demonstrate how to breed Astyanax mexicanus adults, raise the larvae, and perform whole-mount immunohistochemistry on post-larval fish to compare the phenotypes of surface and cave morphotypes.

Tags

Mexican TetraAstyanax MexicanusPost-larval PhenotypesWhole-mount ImmunohistochemistryBreedingLarvaeRotifersNursery ContainersGastrointestinal Tract

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