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In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells
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Journal JoVE Immunologie et infection
In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells

11,672 Views

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10:26 min

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January 20, 2019

DOI:

10.3791/58929-v

DOI:
10.3791/58929-v

•

10:26 min
January 20, 2019

•

19 Views
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1IGH, CNRS,Univ Montpellier, 2Laboratory for Monitoring Innovative Therapies, Department of Biological Hematology,CHU Montpellier, 3UFR de Médecine,Univ Montpellier

Summary

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Using multi-step culture systems, we report an in vitro B cell to plasma cell differentiation model.

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In Vitro DifferentiationHuman Normal Memory B CellsLong-lived Plasma CellsPlasma Cell DifferentiationB2 Plasma Cell DifferentiationTranscriptional ChangesPhenotypeBioinformatic ToolsMemory B Cell PurificationCD40 LigandAnti-polyhistidine Monoclonal Antibody

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Cite this Article

Jourdan, M., de Boussac, H., Viziteu, E., Kassambara, A., Moreaux, J. In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells. J. Vis. Exp. (143), e58929, doi:10.3791/58929 (2019).

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