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利用自动高通量微流控系统对铜绿假单胞菌剪切应力的活细胞分析

JoVE Journal
Bioengineering

This content is Free Access.

JoVE Journal Bioengineering

Live Cell Analysis of Shear Stress on Pseudomonas aeruginosa Using an Automated Higher-Throughput Microfluidic System

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利用自动高通量微流控系统对铜绿假单胞菌剪切应力的活细胞分析

DOI:

10.3791/58926-v

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09:12 min

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January 16, 2019

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Arin L. Sutlief*¹, Helena Valquier-Flynn, Christina Wilson, Marco Perez, Hunter Kleinschmidt, Brett J. Schofield, Elizabeth Delmain, Andrea E. Holmes, Christopher D. Wentworth*⁴

¹Department of Chemistry,Doane University, ²Department of Biology,Doane University, ³Department of Pathology and Microbiology,University of Nebraska Medical Center, ⁴Department of Physics and Engineering,Doane University

Chapters

00:04Title
00:47Equipment Startup
01:27Microfluidic Plate Priming and Seeding
03:39Software and Timed Biofilm Growth Experiment Setup
07:02Results: Representative Percent Threshold Area, Total Biofilm Accumulation, Roughness Coefficient, and Textural Entropy
08:12Conclusion

Summary

Automatic Translation

在这里, 我们描述了使用高通量微流体生物反应器结合荧光显微镜来分析剪切应力对表达绿色荧光蛋白的铜绿假单胞菌生物膜的剪切应力影响, 包括仪器建立了生物膜覆盖、生长速率和形态特性的测定。

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