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Live Cell Analysis of Shear Stress on Pseudomonas aeruginosa Using an Automated Higher-Throughput Microfluidic System
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January 16, 2019
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Here, we describe the use of a higher-throughput microfluidic bioreactor coupled with a fluorescent microscope for the analysis of shear stress effects on Pseudomonas aeruginosa biofilms expressing green fluorescent proteins, including instrument set up, the determination of biofilm coverage, growth rate, and morphological properties.
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Live Cell AnalysisShear StressPseudomonas AeruginosaAutomated Higher-throughput Microfluidic SystemBiofilm DevelopmentEnvironmental ConditionsBiofilm GrowthBehavioral CharacteristicsMulti-channeled Microfluidic PlatesStatistically Significant ResultsBiofilm StructureAntibiotic TreatmentsBioremediationProtocolFluorescence ModuleImaging System ControllerCCD CameraImaging StationMinimal MediumLuria-Bertani BrothShear Stress
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Sutlief, A. L., Valquier-Flynn, H., Wilson, C., Perez, M., Kleinschmidt, H., Schofield, B. J., Delmain, E., Holmes, A. E., Wentworth, C. D. Live Cell Analysis of Shear Stress on Pseudomonas aeruginosa Using an Automated Higher-Throughput Microfluidic System. J. Vis. Exp. (143), e58926, doi:10.3791/58926 (2019).
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