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Preparation of Rhythmically-active In Vitro Neonatal Rodent Brainstem-spinal Cord and Thin Slice
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Neuroscience
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JoVE Journal Neuroscience
Preparation of Rhythmically-active In Vitro Neonatal Rodent Brainstem-spinal Cord and Thin Slice

Preparation of Rhythmically-active In Vitro Neonatal Rodent Brainstem-spinal Cord and Thin Slice

English

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11,301 Views

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06:32 min

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March 23, 2019

DOI:

10.3791/58870-v

DOI:
10.3791/58870-v

•

06:32 min
March 23, 2019

•

9 Views
, , ,
1Center for Perinatal Biology, Department of Basic Sciences,Loma Linda University, 2Department of Biology,The College of New Jersey, 3Department of Neurosurgery,Loma Linda University

Summary

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This protocol both visually communicates the brainstem-spinal cord preparation and clarifies the preparation of brainstem transverse slices in a comprehensive step-by-step manner. It was designed to increase reproducibility and enhance the likelihood of obtaining viable, long lasting, rhythmically-active slices for recording neural output from the respiratory regions of the brainstem.

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Cite this Article

Palahnuk, S. B., Abdala, J. A., Gospodarev, V. V., Wilson, C. G. Preparation of Rhythmically-active In Vitro Neonatal Rodent Brainstem-spinal Cord and Thin Slice. J. Vis. Exp. (145), e58870, doi:10.3791/58870 (2019).

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