/
/
Preparation of Rhythmically-active In Vitro Neonatal Rodent Brainstem-spinal Cord and Thin Slice
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Preparation of Rhythmically-active In Vitro Neonatal Rodent Brainstem-spinal Cord and Thin Slice
11,301 Views
•
06:32 min
•
March 23, 2019
DOI:
Summary
Automatically generated
This protocol both visually communicates the brainstem-spinal cord preparation and clarifies the preparation of brainstem transverse slices in a comprehensive step-by-step manner. It was designed to increase reproducibility and enhance the likelihood of obtaining viable, long lasting, rhythmically-active slices for recording neural output from the respiratory regions of the brainstem.
Read Article
Cite this Article
Palahnuk, S. B., Abdala, J. A., Gospodarev, V. V., Wilson, C. G. Preparation of Rhythmically-active In Vitro Neonatal Rodent Brainstem-spinal Cord and Thin Slice. J. Vis. Exp. (145), e58870, doi:10.3791/58870 (2019).
Copy