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Lipid Bilayer Experiments with Contact Bubble Bilayers for Patch-Clampers
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Lipid Bilayer Experiments with Contact Bubble Bilayers for Patch-Clampers

Lipid Bilayer Experiments with Contact Bubble Bilayers for Patch-Clampers

English

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9,319 Views

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07:18 min

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January 16, 2019

DOI:

10.3791/58840-v

DOI:
10.3791/58840-v

•

07:18 min
January 16, 2019

•

4 Views
,
1Department of Molecular Physiology and Biophysics,University of Fukui Faculty of Medical Sciences

Summary

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Here, we present a protocol for the formation of lipid bilayers using a contact bubble bilayer method. A water bubble is blown into an organic solvent, whereby a monolayer is formed at the water-oil interface. Two pipettes are manipulated to dock the bubbles to form a bilayer.

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Cite this Article

Iwamoto, M., Oiki, S. Lipid Bilayer Experiments with Contact Bubble Bilayers for Patch-Clampers. J. Vis. Exp. (143), e58840, doi:10.3791/58840 (2019).

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