ジャーナル
/
神経科学
/
Lipid Bilayer Experiments with Contact Bubble Bilayers for Patch-Clampers
JoVE Journal
神経科学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 神経科学
Lipid Bilayer Experiments with Contact Bubble Bilayers for Patch-Clampers

Lipid Bilayer Experiments with Contact Bubble Bilayers for Patch-Clampers

DOI:
10.3791/58840-v

•

07:18 min

•

January 16, 2019

•

,
1Department of Molecular Physiology and Biophysics,University of Fukui Faculty of Medical Sciences

章

  • 00:04標題
  • 00:58Liposomes and Pipettes Preparation
  • 02:41Electrophysiological Measurement
  • 05:49Results: Detach-attach of Monolayers and Responses of Channels
  • 06:37Conclusion

概要

自動翻訳

自動翻訳

Here, we present a protocol for the formation of lipid bilayers using a contact bubble bilayer method. A water bubble is blown into an organic solvent, whereby a monolayer is formed at the water-oil interface. Two pipettes are manipulated to dock the bubbles to form a bilayer.

タグ

Lipid BilayerContact Bubble BilayerPatch-clampingPlanar Lipid BilayerMembrane VibrationSingle Channel CurrentPhospholipid SuspensionProteoliposomesIon Channel ProteinsGlass PipettesMicro ForgeSiliconizing ReagentInverted Microscope

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Functional Mapping with Simultaneous MEG and EEG

Combining Computer Game-Based Behavioural Experiments With High-Density EEG and Infrared Gaze Tracking

Examining Local Network Processing using Multi-contact Laminar Electrode Recording

Closed-loop Neuro-robotic Experiments to Test Computational Properties of Neuronal Networks

SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy

Fabrication of High Contact-Density, Flat-Interface Nerve Electrodes for Recording and Stimulation Applications

Preparing Fresh Retinal Slices from Adult Zebrafish for Ex Vivo Imaging Experiments

Conducting Hyperscanning Experiments with Functional Near-Infrared Spectroscopy

Using Neuron Spiking Activity to Trigger Closed-Loop Stimuli in Neurophysiological Experiments

Generation of Oligodendrocytes and Oligodendrocyte-Conditioned Medium for Co-Culture Experiments

Read Article