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Lipid Bilayer Experiments with Contact Bubble Bilayers for Patch-Clampers
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Lipid Bilayer Experiments with Contact Bubble Bilayers for Patch-Clampers
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January 16, 2019
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Here, we present a protocol for the formation of lipid bilayers using a contact bubble bilayer method. A water bubble is blown into an organic solvent, whereby a monolayer is formed at the water-oil interface. Two pipettes are manipulated to dock the bubbles to form a bilayer.
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Iwamoto, M., Oiki, S. Lipid Bilayer Experiments with Contact Bubble Bilayers for Patch-Clampers. J. Vis. Exp. (143), e58840, doi:10.3791/58840 (2019).
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