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用于补丁夹紧器的接触气泡层的脂质双层实验

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Lipid Bilayer Experiments with Contact Bubble Bilayers for Patch-Clampers

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用于补丁夹紧器的接触气泡层的脂质双层实验

9,291 Views

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07:18 min

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January 16, 2019

DOI:

10.3791/58840-v

DOI:

10.3791/58840-v

•

07:18 min

January 16, 2019

•

4 Views

Masayuki Iwamoto¹, Shigetoshi Oiki¹

¹Department of Molecular Physiology and Biophysics,University of Fukui Faculty of Medical Sciences

Summary

在这里, 我们提出了一个协议, 形成脂质双层使用接触气泡双层法。水泡被吹入有机溶剂, 藉以单层被形成在水油接口。两个移液器纵 , 以停靠气泡 , 形成一个双层。

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