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Calretinin için Immünostasyon rektal emiş biyopsisi ile Hirschsprung hastalığının tanısı, S100 protein ve protein gen ürün 9,5
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JoVE Journal Neuroscience
Diagnosis of Hirschsprung’s Disease by Immunostaining Rectal Suction Biopsies for Calretinin, S100 Protein and Protein Gene Product 9.5
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Calretinin için Immünostasyon rektal emiş biyopsisi ile Hirschsprung hastalığının tanısı, S100 protein ve protein gen ürün 9,5

Türkçe

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12,918 Views

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05:45 min

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April 26, 2019

DOI:

10.3791/58799-v

DOI:
10.3791/58799-v

•

05:45 min
April 26, 2019

•

13 Views
, , , ,
1Department of Pediatric Surgery, Union Hospital, Tongji Medical College,Huazhong University of Science and Technology, 2Division of Gastroenterology, Hepatology and Nutrition,Cincinnati Children’s Hospital Medical Center

Summary

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Bu protokol, calretinin, S100 proteini ve protein geni ürün 9,5 için immünostasyon rektal emiş biyopsisi sürecini açıklar. Hirschsprung hastalığı için bu roman adjuvan tanı yöntemi tercih duyarlılık ve özgüllük oranları vardır.

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Hirschsprung’s DiseaseImmunostainingRectal Suction BiopsyCalretininS100 ProteinProtein Gene Product 9.5DiagnosisSensitivitySpecificityParaformaldehydeDehydrationParaffin EmbeddingMicrotomeAntigen RetrievalHydrogen PeroxideBovine Serum AlbuminPrimary AntibodyPolymer EnhancerSecondary Antibody

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Cite this Article

Chi, S., Fang, M., Li, K., Yang, L., Tang, S. Diagnosis of Hirschsprung's Disease by Immunostaining Rectal Suction Biopsies for Calretinin, S100 Protein and Protein Gene Product 9.5. J. Vis. Exp. (146), e58799, doi:10.3791/58799 (2019).

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