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Diagnose der Hirschsprung-Krankheit durch die Immunofelflecken-Rectal Suction Biopsien für Calretinin, S100 Protein und Protein Gene Produkt 9.5
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Neuroscience
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JoVE Journal Neuroscience
Diagnosis of Hirschsprung’s Disease by Immunostaining Rectal Suction Biopsies for Calretinin, S100 Protein and Protein Gene Product 9.5
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Diagnose der Hirschsprung-Krankheit durch die Immunofelflecken-Rectal Suction Biopsien für Calretinin, S100 Protein und Protein Gene Produkt 9.5

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12,918 Views

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05:45 min

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April 26, 2019

DOI:

10.3791/58799-v

DOI:
10.3791/58799-v

•

05:45 min
April 26, 2019

•

13 Views
, , , ,
1Department of Pediatric Surgery, Union Hospital, Tongji Medical College,Huazhong University of Science and Technology, 2Division of Gastroenterology, Hepatology and Nutrition,Cincinnati Children’s Hospital Medical Center

Summary

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Dieses Protokoll beschreibt den Prozess der Immunfärbung von rektalen Absaugbiopsien für Calretinin, S100-Protein und Protein-Genprodukt 9.5. Diese neuartige Adjuvan-Diagnostik für Hirschsprungs Krankheit hat bevorzugt Empfindlichkeits-und Spezifitätsraten.

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Hirschsprung’s DiseaseImmunostainingRectal Suction BiopsyCalretininS100 ProteinProtein Gene Product 9.5DiagnosisSensitivitySpecificityParaformaldehydeDehydrationParaffin EmbeddingMicrotomeAntigen RetrievalHydrogen PeroxideBovine Serum AlbuminPrimary AntibodyPolymer EnhancerSecondary Antibody

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Chi, S., Fang, M., Li, K., Yang, L., Tang, S. Diagnosis of Hirschsprung's Disease by Immunostaining Rectal Suction Biopsies for Calretinin, S100 Protein and Protein Gene Product 9.5. J. Vis. Exp. (146), e58799, doi:10.3791/58799 (2019).

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