Journal
/
Neuroscience
/
Effects of Blast-induced Neurotrauma on Pressurized Rodent Middle Cerebral Arteries
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Effects of Blast-induced Neurotrauma on Pressurized Rodent Middle Cerebral Arteries

Effects of Blast-induced Neurotrauma on Pressurized Rodent Middle Cerebral Arteries

DOI:
10.3791/58792-v

•

08:21 min

•

April 01, 2019

•

, , , , ,
1Charles R. Allen Research Laboratories, Department of Anesthesiology,University of Texas Medical Branch, 2The Moody Project for Translational Traumatic Brain Injury Research,University of Texas Medical Branch

Chapters

  • 00:04Title
  • 01:06Advanced Blast Simulator (ABS) Blast Device Preparation
  • 02:06ABS Injury Preparation
  • 03:58Blast-traumatic Brain Injury (TBI) Induction
  • 04:38Rodent Middle Cerebral Artery (MCA) Segment Extraction
  • 07:04Results: Representative Effects of Blast-induced Traumatic Brain Injury (bTBI) on MCA Responses to Reduced Intravascular Pressure
  • 08:00Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol to describe methods for ex vivo vascular reactivity determination following a primary blast traumatic brain injury (bTBI) using isolated, pressurized, rodent middle cerebral arterial (MCA) segments. bTBI induction is accomplished using a shock tube, also known as an Advanced Blast Simulator (ABS) device.

Tags

Blast-induced NeurotraumaPressurized Rodent Middle Cerebral ArteriesCerebral Vascular InjuryMyogenic Vascular ResponseIsolated Pressurized Arterial PreparationAdvanced Blast SimulatorMylar SheetsHydraulic Hand PumpAnesthetized RatEndotracheal TubeVentilatorSpecimen TrayHeat LampScalpEar Plug

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Focal Cerebral Ischemia Model by Endovascular Suture Occlusion of the Middle Cerebral Artery in the Rat

DiOLISTIC Labeling of Neurons from Rodent and Non-human Primate Brain Slices

Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals

Simultaneous Electroencephalography, Real-time Measurement of Lactate Concentration and Optogenetic Manipulation of Neuronal Activity in the Rodent Cerebral Cortex

Isolation of Cerebrospinal Fluid from Rodent Embryos for use with Dissected Cerebral Cortical Explants

Lateral Chronic Cranial Window Preparation Enables In Vivo Observation Following Distal Middle Cerebral Artery Occlusion in Mice

Isolation of Cerebral Capillaries from Fresh Human Brain Tissue

Quantitative Autoradiographic Method for Determination of Regional Rates of Cerebral Protein Synthesis In Vivo

Modeling Stroke in Mice: Transient Middle Cerebral Artery Occlusion via the External Carotid Artery

A Model for Encephalomyosynangiosis Treatment after Middle Cerebral Artery Occlusion-Induced Stroke in Mice

Read Article